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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
2000-8-16
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pubmed:databankReference |
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pubmed:abstractText |
We identified a unique type II cAMP-dependent protein kinase regulatory subunit (PKA-RII) gene in Drosophila melanogaster and a severely hypomorphic if not null mutation, pka-RII(EP(2)2162). Extracts from pka- RII(EP(2)2162) flies selectively lack RII-specific autophosphorylation activity and show significantly reduced cAMP binding activity, attributable to the loss of functional PKA-RII. pka-RII(EP(2)2162) shows 2-fold increased basal PKA activity and approximately 40% of normal cAMP-inducible PKA activity. pka-RII(EP(2)2162) is fully viable but displays abnormalities of ovarian development and multiple behavioral phenotypes including arrhythmic circadian locomotor activity, decreased sensitivity to ethanol and cocaine, and a lack of sensitization to repeated cocaine exposures. These findings implicate type II PKA activity in these processes in Drosophila and imply a common role for PKA signaling in regulating responsiveness to cocaine and alcohol.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20588-96
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10781603-Amino Acid Sequence,
pubmed-meshheading:10781603-Animals,
pubmed-meshheading:10781603-Circadian Rhythm,
pubmed-meshheading:10781603-Cloning, Molecular,
pubmed-meshheading:10781603-Cocaine,
pubmed-meshheading:10781603-Cyclic AMP,
pubmed-meshheading:10781603-Cyclic AMP-Dependent Protein Kinase Type II,
pubmed-meshheading:10781603-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:10781603-Drosophila melanogaster,
pubmed-meshheading:10781603-Ethanol,
pubmed-meshheading:10781603-Microscopy, Fluorescence,
pubmed-meshheading:10781603-Molecular Sequence Data,
pubmed-meshheading:10781603-Mutation,
pubmed-meshheading:10781603-Phenotype,
pubmed-meshheading:10781603-Phosphorylation,
pubmed-meshheading:10781603-Reproduction,
pubmed-meshheading:10781603-Sequence Homology, Amino Acid
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pubmed:year |
2000
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pubmed:articleTitle |
Type II cAMP-dependent protein kinase-deficient Drosophila are viable but show developmental, circadian, and drug response phenotypes.
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pubmed:affiliation |
Department of Biology, University of Virginia, Charlottesville, Virginia 22903, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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