Source:http://linkedlifedata.com/resource/pubmed/id/10774930
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2000-6-19
|
| pubmed:abstractText |
Zinc plays an important role in the maintenance of the immune system. While the mechanisms of zinc ions interaction with immune cells are still poorly understood, a striking concurrent effect of zinc is the induction of the biosynthesis of metallothioneins (MT), a group of low molecular weight, cysteine-rich metal-binding proteins, believed to play a role in zinc homeostasis. In humans, they are encoded by a family of genes, located at 16q13 containing 10 functional and 7 non-functional MT isoforms. In this work we analyzed the spectrum of different isoforms in human peripheral blood lymphocytes. It was demonstrated by RT-PCR that the MT-2a, MT-1a, MT-1e, MT-1f, MT-1g, MT-1h and MT-1x genes are expressed in these cells and that these isoforms are further upregulated by zinc, as examined by quantitative RT-PCR. Surprisingly, RT-PCR also showed the presence, even in unstimulated cells, of MT-3 transcripts, which are considered as brain-specific isoforms. In an effort to determine whether MTmRNA abundance is translated into MT protein, MT isolated from zinc-treated lymphocytes by gel chromatography was resolved into 7 metal-binding fractions by using RP-HPLC. Automatic Edman-degradation of the different fractions revealed the presence of MT-2a, MT-1a, MT-1e, MT-1f, MT-1g, MT-1h, MT-1x and MT-1k, an isoform which until now was only identified at the level of protein in human liver and kidney tissue.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadmium,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0145-5680
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
419-33
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10774930-Amino Acid Sequence,
pubmed-meshheading:10774930-Cadmium,
pubmed-meshheading:10774930-Cells, Cultured,
pubmed-meshheading:10774930-Chromatography, High Pressure Liquid,
pubmed-meshheading:10774930-DNA Primers,
pubmed-meshheading:10774930-Electrophoresis, Agar Gel,
pubmed-meshheading:10774930-Hemoglobins,
pubmed-meshheading:10774930-Humans,
pubmed-meshheading:10774930-Lymphocytes,
pubmed-meshheading:10774930-Metallothionein,
pubmed-meshheading:10774930-Molecular Sequence Data,
pubmed-meshheading:10774930-Protein Binding,
pubmed-meshheading:10774930-Protein Isoforms,
pubmed-meshheading:10774930-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10774930-Sequence Homology, Amino Acid,
pubmed-meshheading:10774930-Time Factors,
pubmed-meshheading:10774930-Transcription, Genetic,
pubmed-meshheading:10774930-Zinc
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Metallothionein isoform gene expression in zinc-treated human peripheral blood lymphocytes.
|
| pubmed:affiliation |
Laboratorium voor Biochemie, Katholieke Universiteit Leuven, Heverlee, Belgium.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|