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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2000-5-31
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pubmed:databankReference |
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pubmed:abstractText |
A Drosophila cDNA encoding a structural homolog of mammalian FKBP59 (also identified as FKBP52), a member of the FK506-binding protein (FKBP) class of immunophilins, was isolated. The gene dFKBP59 corresponding to this cDNA has been characterized and mapped to the 30D3-4 region. The predicted amino acid sequence of this cDNA shows that the dFKBP59 protein contains one highly conserved FKBP12-like domain followed by two others with less conservation. Northern hybridization reveals that the dFKBP59 mRNA is expressed throughout the Drosophila life-cycle. In contrast to its mammalian homologs, in situ hybridization detected dFKBP59 expression in specific tissues: the lymph glands, Garland cells and oenocyte cells, which are all specialized tissues in which intensive exocytic/endocytic cycling takes place. Garland cells and oenocytes (also called Drosophila nephrocytes) function in taking up waste material from the hemolymph. Finally, I have mapped an enhancer trap element within the 5' region of dFKBP59 which may help in future studies to address the question of its function during Drosophila development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
246
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10767531-Amino Acid Sequence,
pubmed-meshheading:10767531-Animals,
pubmed-meshheading:10767531-Chromosome Mapping,
pubmed-meshheading:10767531-Cloning, Molecular,
pubmed-meshheading:10767531-DNA,
pubmed-meshheading:10767531-DNA, Complementary,
pubmed-meshheading:10767531-DNA Transposable Elements,
pubmed-meshheading:10767531-Drosophila,
pubmed-meshheading:10767531-Embryo, Nonmammalian,
pubmed-meshheading:10767531-Embryonic Development,
pubmed-meshheading:10767531-Exons,
pubmed-meshheading:10767531-Female,
pubmed-meshheading:10767531-Gene Expression Regulation, Developmental,
pubmed-meshheading:10767531-Genes, Insect,
pubmed-meshheading:10767531-Immunophilins,
pubmed-meshheading:10767531-In Situ Hybridization,
pubmed-meshheading:10767531-Introns,
pubmed-meshheading:10767531-Male,
pubmed-meshheading:10767531-Molecular Sequence Data,
pubmed-meshheading:10767531-Mutagenesis, Insertional,
pubmed-meshheading:10767531-RNA, Messenger,
pubmed-meshheading:10767531-Sequence Alignment,
pubmed-meshheading:10767531-Sequence Analysis, DNA,
pubmed-meshheading:10767531-Sequence Homology, Amino Acid,
pubmed-meshheading:10767531-Tacrolimus Binding Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Molecular cloning and embryonic expression of dFKBP59, a novel Drosophila FK506-binding protein.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA. stephane_zaffran@smtplink.mssm.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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