Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-5-4
pubmed:abstractText
Here we show that vascular endothelial growth factor (VEGF) mRNA expression is up-regulated in oncogene transformed rat liver epithelial (RLE) cell lines and that the extracellular signal-regulated kinase (ERK) and p38 kinase differentially regulate the oncogene-mediated stimulation of VEGF. The highest level of VEGF mRNA expression was observed in the v-H-ras transformed RLE cell line, followed by the v-raf and v-myc transformed lines. The PD98059 MEK inhibitor was used to block the ERK pathway and SB203580 inhibitor to block the p38 pathway. The parent and the v-H-ras transformed RLE cell lines showed up-regulation of VEGF RNA expression through the ERK pathway and down-regulation of VEGF through the p38 pathway. VEGF was regulated in a comparable manner in a human breast carcinoma cell line. In the v-raf and v-myc transformed RLE lines, positive regulation of VEGF was transduced through the p38 pathway. These findings suggest that (1) oncogenic ras differs from raf and myc in the recruitment of the MAPK signaling pathways for VEGF regulation; (2) that VEGF is regulated in ras transformed and human cancer cell lines in a positive and negative manner by the ERK and p38 signaling pathways.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent..., http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors, http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids, http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles, http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins v-raf, http://linkedlifedata.com/resource/pubmed/chemical/PD 98059, http://linkedlifedata.com/resource/pubmed/chemical/Pyridines, http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic, http://linkedlifedata.com/resource/pubmed/chemical/SB 203580, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A, http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors, http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
108-11
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10733912-Animals, pubmed-meshheading:10733912-Breast Neoplasms, pubmed-meshheading:10733912-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:10733912-Carcinoma, pubmed-meshheading:10733912-Cell Line, pubmed-meshheading:10733912-Cell Transformation, Neoplastic, pubmed-meshheading:10733912-Endothelial Growth Factors, pubmed-meshheading:10733912-Epithelial Cells, pubmed-meshheading:10733912-Flavonoids, pubmed-meshheading:10733912-Genes, myc, pubmed-meshheading:10733912-Genes, ras, pubmed-meshheading:10733912-Imidazoles, pubmed-meshheading:10733912-Liver, pubmed-meshheading:10733912-Lymphokines, pubmed-meshheading:10733912-MAP Kinase Signaling System, pubmed-meshheading:10733912-Mitogen-Activated Protein Kinases, pubmed-meshheading:10733912-Neovascularization, Pathologic, pubmed-meshheading:10733912-Oncogene Proteins v-raf, pubmed-meshheading:10733912-Pyridines, pubmed-meshheading:10733912-Rats, pubmed-meshheading:10733912-Retroviridae Proteins, Oncogenic, pubmed-meshheading:10733912-Vascular Endothelial Growth Factor A, pubmed-meshheading:10733912-Vascular Endothelial Growth Factors, pubmed-meshheading:10733912-p38 Mitogen-Activated Protein Kinases
pubmed:year
2000
pubmed:articleTitle
Different regulation of vascular endothelial growth factor expression by the ERK and p38 kinase pathways in v-ras, v-raf, and v-myc transformed cells.
pubmed:affiliation
Tumor Biology and Carcinogenesis Section, Laboratory of Cellular Carcinogenesis and Tumor Promotion, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article, Comparative Study