Linked Life Data

10733549

Source:http://linkedlifedata.com/resource/pubmed/id/10733549

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-4-19
pubmed:abstractText
Liver myofibroblasts are major actors in the development of liver fibrosis and cancer progression. There is a large interest in drugs that might deactivate these cells. Many studies have shown that the grapevine-derived polyphenol, trans-resveratrol, and other stilbenes have therapeutic potential in some diseases. In this work, we have studied the effect of grapevine polyphenols on cultured human liver myofibroblasts. We have shown that trans-resveratrol profoundly affects myofibroblast phenotype. Trans-resveratrol induced morphological modifications. It markedly reduced proliferation of myofibroblasts in a dose-dependent manner. Trans-resveratrol also decreased the expression of alpha smooth muscle actin (alpha-SMA) without affecting vimentin or beta-cytoplasmic actin expression. It decreased myofibroblast migration in a monolayer wounding assay. We also showed that trans-resveratrol inhibited the messenger RNA (mRNA) expression of type I collagen. Finally, it decreased the secretion of matrix metalloproteinase 2 (MMP-2). We conclude that trans-resveratrol can deactivate human liver myofibroblasts. In the second part of this study, we have shown that neither trans-piceid (a glycosylated analog) nor trans-piceatannol (a hydroxylated analog) reproduces trans-resveratrol effects on liver myofibroblasts. We finally show that, although trans-resveratrol decreases the proliferation of skin fibroblast and vascular smooth muscle cells, it does not affect their expression of alpha-SMA, which indicates some cell specificity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
922-31
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10733549-Actins, pubmed-meshheading:10733549-Antioxidants, pubmed-meshheading:10733549-Cell Division, pubmed-meshheading:10733549-Cell Movement, pubmed-meshheading:10733549-Cells, Cultured, pubmed-meshheading:10733549-Enzyme Activation, pubmed-meshheading:10733549-Fibroblasts, pubmed-meshheading:10733549-Gene Expression, pubmed-meshheading:10733549-Humans, pubmed-meshheading:10733549-Liver, pubmed-meshheading:10733549-Matrix Metalloproteinase 2, pubmed-meshheading:10733549-Microscopy, Electron, pubmed-meshheading:10733549-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:10733549-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:10733549-Mitogen-Activated Protein Kinases, pubmed-meshheading:10733549-Muscle, Smooth, Vascular, pubmed-meshheading:10733549-Procollagen, pubmed-meshheading:10733549-RNA, Messenger, pubmed-meshheading:10733549-Stem Cells, pubmed-meshheading:10733549-Stilbenes
pubmed:year
2000
pubmed:articleTitle
Deactivation of cultured human liver myofibroblasts by trans-resveratrol, a grapevine-derived polyphenol.
pubmed:affiliation
Groupe de Recherches pour l'Etude du Foie INSERM E9917, Faculté des Sciences Pharmaceutiques, Université Victor Segalen Bordeaux 2, Bordeaux, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't