Linked Life Data

10671504

Source:http://linkedlifedata.com/resource/pubmed/id/10671504

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-3-21
pubmed:abstractText
Sterol regulatory element-binding proteins (SREBPs) activate genes of cholesterol and fatty acid metabolism. In each case, a ubiquitous co-regulatory factor that binds to a neighboring recognition site is also required for efficient promoter activation. It is likely that gene- and pathway-specific regulation by the separate SREBP isoforms is dependent on subtle differences in how the individual proteins function with specific co-regulators to activate gene expression. In the studies reported here we extend these observations significantly by demonstrating that SREBPs are involved in both sterol regulation and carbohydrate activation of the FAS promoter. We also demonstrate that the previously implicated Sp1 site is largely dispensable for sterol regulation in established cultured cells, whereas a CCAAT-binding factor/nuclear factor Y is critically important. In contrast, carbohydrate activation of the FAS promoter in primary hepatocytes is dependent upon SREBP and both the Sp1 and CCAAT-binding factor/nuclear factor Y sites. Because 1c is the predominant SREBP isoform expressed in hepatocytes and 1a is more abundant in sterol depleted established cell lines, this suggests that the different SREBP isoforms utilize distinct co-regulatory factors to activate target gene expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4726-33
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10671504-Animals, pubmed-meshheading:10671504-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:10671504-Cell Line, pubmed-meshheading:10671504-DNA Primers, pubmed-meshheading:10671504-DNA-Binding Proteins, pubmed-meshheading:10671504-Drosophila, pubmed-meshheading:10671504-Fatty Acid Synthetase Complex, pubmed-meshheading:10671504-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10671504-Male, pubmed-meshheading:10671504-Mutation, pubmed-meshheading:10671504-Nuclear Proteins, pubmed-meshheading:10671504-Promoter Regions, Genetic, pubmed-meshheading:10671504-Protein Isoforms, pubmed-meshheading:10671504-Rats, pubmed-meshheading:10671504-Rats, Sprague-Dawley, pubmed-meshheading:10671504-Sterol Regulatory Element Binding Protein 1, pubmed-meshheading:10671504-Transcription Factors
pubmed:year
2000
pubmed:articleTitle
Different sterol regulatory element-binding protein-1 isoforms utilize distinct co-regulatory factors to activate the promoter for fatty acid synthase.
pubmed:affiliation
Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't