Linked Life Data

10659681

Source:http://linkedlifedata.com/resource/pubmed/id/10659681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2000-2-18
pubmed:abstractText
Neurons are terminally post-mitotic cells that utilize their microtubule arrays for the growth and maintenance of axons and dendrites rather than for the formation of mitotic spindles. Recent studies from our laboratory suggest that the mechanisms that organize the axonal and dendritic microtubule arrays may be variations on the same mechanisms that organize the mitotic spindle in dividing cells. In particular, we have identified molecular motor proteins that serve analogous functions in the establishment of these seemingly very different microtubule arrays. In the present study, we have sought to determine whether a non-motor protein termed NuMA is also a component of both systems. NuMA is a approximately 230 kDa structural protein that is present exclusively in the nucleus during interphase. During mitosis, NuMA forms aggregates that interact with microtubules and certain motor proteins. As a result of these interactions, NuMA is thought to draw together the minus-ends of microtubules, thereby helping to organize them into a bipolar spindle. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. NuMA appears as multiple small particles within the somatodendritic compartment of the neuron, where its levels increase during early dendritic differentiation. A partial but not complete colocalization with minus-ends of microtubules is suggested by the distribution of the particles during development and during drug treatments that alter the microtubule array. These observations provide an initial set of clues regarding a potentially important function of NuMA in the organization of microtubules within the somatodendritic compartment of the neuron.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0300-4864
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
887-99
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10659681-Animals, pubmed-meshheading:10659681-Antigens, Nuclear, pubmed-meshheading:10659681-Axons, pubmed-meshheading:10659681-Bucladesine, pubmed-meshheading:10659681-Cell Cycle, pubmed-meshheading:10659681-Cells, Cultured, pubmed-meshheading:10659681-Coculture Techniques, pubmed-meshheading:10659681-Cytochalasin B, pubmed-meshheading:10659681-Dendrites, pubmed-meshheading:10659681-Embryo, Mammalian, pubmed-meshheading:10659681-HeLa Cells, pubmed-meshheading:10659681-Hippocampus, pubmed-meshheading:10659681-Humans, pubmed-meshheading:10659681-Kinetics, pubmed-meshheading:10659681-Microtubules, pubmed-meshheading:10659681-Mitotic Spindle Apparatus, pubmed-meshheading:10659681-Neuroblastoma, pubmed-meshheading:10659681-Neurons, pubmed-meshheading:10659681-Nocodazole, pubmed-meshheading:10659681-Nuclear Matrix-Associated Proteins, pubmed-meshheading:10659681-Nuclear Proteins, pubmed-meshheading:10659681-Paclitaxel, pubmed-meshheading:10659681-Rats, pubmed-meshheading:10659681-Sympathetic Nervous System, pubmed-meshheading:10659681-Tumor Cells, Cultured
pubmed:year
1998
pubmed:articleTitle
The nuclear/mitotic apparatus protein NuMA is a component of the somatodendritic microtubule arrays of the neuron.
pubmed:affiliation
Department of Anatomy, University of Wisconsin Medical School, Madison 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't