Linked Life Data

10656487

Source:http://linkedlifedata.com/resource/pubmed/id/10656487

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-2-17
pubmed:abstractText
The role of a defect for nucleotide excision repair (NER) in oocytes on the repair of DNA ethyl adducts induced by diethyl sulfate (DES) in male germ cells of Drosophila was analysed. Frequencies of mutations at multiple loci (recessive lethal mutations) and at the vermilion gene induced in NER+ conditions (cross NER+ x NER+) were compared with those fixed in a NER- background (NER- x NER+). The M(NER-)/M(NER+) mutability ratios for two DES concentrations, 10 mM and 15 mM, were 2.21 and 1.49, respectively, indicating that NER repairs part of the DES-induced damage. The majority of 28 fertile vermilion mutations produced by DES in NER- are transitions, both GC-AT (46.4%) and AT-GC (21.4%) transitions are found, the consequences of O6-ethylguanine and O4-ethylthymine, respectively. Transversions (21.5%), one +1 frameshift mutation (3.6%) and two deletions (7.1%) are most likely the result of N-alkylation damage. Furthermore, the DES-induced mutation spectra show interesting differences in relation to the exposure dose. All 10 mutants isolated in this and a previous [L.M. Sierra, A. Pastink, M.J.M. Nivard, E.W. Vogel, DNA base sequence changes induced by DES in postmeiotic male germ cells of Drosophila melanogaster, Mol. Gen. Genet. 237 (1993) 370-374] study from experiments with low DES-effectiveness are exclusively transitions, independent whether the females were of the NER+ or NER-genotype. This indicates that at lower DES effectiveness only O-alkylation damage is relevant, and that N-alkylation damage is repaired. In experiments revealing high DES-effectiveness, vermilion mutations representing N-alkylation damage reached 43% (9/21) with NER- and 26% (7/27) with NER+ females, suggesting (i) that NER becomes involved at high adduct levels because then the base excision repair (BER) may be saturated, and (ii) that this involvement of NER causes the relative decrease from 43% to 26% N-alkylation mediated sequence changes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
431
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-79
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10656487-Alkylating Agents, pubmed-meshheading:10656487-Animals, pubmed-meshheading:10656487-DNA Repair, pubmed-meshheading:10656487-Dose-Response Relationship, Drug, pubmed-meshheading:10656487-Drosophila, pubmed-meshheading:10656487-Drosophila Proteins, pubmed-meshheading:10656487-Eye Proteins, pubmed-meshheading:10656487-Female, pubmed-meshheading:10656487-Genes, Lethal, pubmed-meshheading:10656487-Genes, Recessive, pubmed-meshheading:10656487-Insect Proteins, pubmed-meshheading:10656487-Male, pubmed-meshheading:10656487-Meiosis, pubmed-meshheading:10656487-Mutagens, pubmed-meshheading:10656487-Mutation, pubmed-meshheading:10656487-Sequence Analysis, DNA, pubmed-meshheading:10656487-Spermatozoa, pubmed-meshheading:10656487-Sulfuric Acid Esters, pubmed-meshheading:10656487-Tryptophan Oxygenase
pubmed:year
1999
pubmed:articleTitle
Influence of nucleotide excision repair and of dose on the types of vermilion mutations induced by diethyl sulfate in postmeiotic male germ cells of Drosophila.
pubmed:affiliation
Departamento de Biología Funcional, Area de Genética, University of Oviedo, Spain. lmsierra@correo.uniovi.es
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't