Source:http://linkedlifedata.com/resource/pubmed/id/10612750
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2000-3-20
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| pubmed:databankReference | |
| pubmed:abstractText |
The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft-1 and bft-2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft-1, and bft-2 genes were determined for five, four, and four ETBF strains, respectively; the nucleotide sequence was identical among each bft subtype and the degree of identity between each subtype was between 89 and 94%. Most of the variations between the three subtypes were detected in the region encoding mature toxin. A multiplex PCR was developed with a four-primer mix to subtype the bft sequences. The subtyping of 143 ETBF isolates from extraintestinal and stool specimens of humans and cows showed that the bft-1 was the most prevalent subtype, followed by bft-2 and a new bft subtype. No other subtype was found among the strains tested.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
0378-1097
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
182
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
171-6
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10612750-Adolescent,
pubmed-meshheading:10612750-Adult,
pubmed-meshheading:10612750-Aged,
pubmed-meshheading:10612750-Amino Acid Sequence,
pubmed-meshheading:10612750-Animals,
pubmed-meshheading:10612750-Bacterial Toxins,
pubmed-meshheading:10612750-Bacteroides Infections,
pubmed-meshheading:10612750-Bacteroides fragilis,
pubmed-meshheading:10612750-Base Sequence,
pubmed-meshheading:10612750-Cattle,
pubmed-meshheading:10612750-Child,
pubmed-meshheading:10612750-Child, Preschool,
pubmed-meshheading:10612750-Diarrhea,
pubmed-meshheading:10612750-Feces,
pubmed-meshheading:10612750-Humans,
pubmed-meshheading:10612750-Infant,
pubmed-meshheading:10612750-Infant, Newborn,
pubmed-meshheading:10612750-Japan,
pubmed-meshheading:10612750-Metalloendopeptidases,
pubmed-meshheading:10612750-Middle Aged,
pubmed-meshheading:10612750-Molecular Sequence Data,
pubmed-meshheading:10612750-Polymerase Chain Reaction,
pubmed-meshheading:10612750-Sequence Analysis, DNA
|
| pubmed:year |
2000
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| pubmed:articleTitle |
A new subtype of the metalloprotease toxin gene and the incidence of the three bft subtypes among Bacteroides fragilis isolates in Japan.
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| pubmed:affiliation |
Institute of Anaerobic Bacteriology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu, Japan. nk19@cc.gifu-u.ac.jp
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| pubmed:publicationType |
Journal Article
|