Linked Life Data

10601973

Source:http://linkedlifedata.com/resource/pubmed/id/10601973

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-2-24
pubmed:abstractText
The coexpression of IGF (-I and -II) peptides, corresponding receptors, and IGF binding proteins (IGFBPs) in uterine endometrium suggests that a significant component of IGF action in this tissue is via autocrine or paracrine pathways, or both. The present study examined whether IGF-II and a major uterine-expressed IGF-II binding protein, IGFBP-2, modulate endometrial epithelial cell mitogenesis. Serum-deprived porcine endometrial glandular epithelial (GE) cells of early pregnancy were treated with various concentrations of IGFs, recombinant porcine (rp) IGFBP-2, or both, and examined for changes in cellular mitogenesis by incorporation of [(3)H]thymidine into DNA. Recombinant human (rh) IGF-II stimulated DNA synthesis in a dose-dependent manner. Human [Leu(27)]-IGF-II, an analog with selective affinity for the IGF-II (type II) receptor, increased thymidine uptake by twofold compared with untreated GE cells. When added in combination with an equimolar concentration of rhIGF-I, [Leu(27)]-IGF-II or rhIGF-II stimulated thymidine incorporation to a greater extent than did rhIGF-I alone. Ligand blot analysis of GE cell conditioned medium revealed the presence of four IGFBPs with molecular masses of 48, 31, 23, and 15 kDa. Physiological concentrations of rpIGFBP-2 (nM range) increased both basal and IGF-induced DNA synthesis in GE cells. At equimolar concentrations, Des(1-6)IGF-II (an IGF-II analog with much reduced affinity for IGFBPs) and rpIGFBP-2 had additive effects on GE cell mitogenesis, suggesting that the IGFBP-2 modulation of uterine cell growth may involve both IGF-dependent and IGF-independent pathways. Our results demonstrate the complex interplay of IGF system components in uterine endometrial epithelial growth regulation in vitro, identify IGF-II and IGFBP-2 as locally coexpressed uterine epithelial cell mitogens, and suggest the presence of a functional signaling pathway by which IGF-II stimulates epithelial cell proliferation via the type II receptor.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0952-5041
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10601973-Animals, pubmed-meshheading:10601973-Cell Division, pubmed-meshheading:10601973-Cells, Cultured, pubmed-meshheading:10601973-Culture Media, Conditioned, pubmed-meshheading:10601973-DNA, pubmed-meshheading:10601973-Dose-Response Relationship, Drug, pubmed-meshheading:10601973-Endometrium, pubmed-meshheading:10601973-Epithelial Cells, pubmed-meshheading:10601973-Female, pubmed-meshheading:10601973-Humans, pubmed-meshheading:10601973-Insulin-Like Growth Factor Binding Protein 2, pubmed-meshheading:10601973-Insulin-Like Growth Factor I, pubmed-meshheading:10601973-Insulin-Like Growth Factor II, pubmed-meshheading:10601973-Mitogens, pubmed-meshheading:10601973-Molecular Weight, pubmed-meshheading:10601973-Peptides, pubmed-meshheading:10601973-Pregnancy, pubmed-meshheading:10601973-RNA, Messenger, pubmed-meshheading:10601973-Recombinant Fusion Proteins, pubmed-meshheading:10601973-Swine
pubmed:year
1999
pubmed:articleTitle
Complex mediation of uterine endometrial epithelial cell growth by insulin-like growth factor-II (IGF-II) and IGF-binding protein-2.
pubmed:affiliation
Interdisciplinary Concentration in Animal Molecular and Cell Biology, Department of Animal Science, University of Florida, Gainesville, Florida 32611, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't