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rdf:type |
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lifeskim:mentions |
umls-concept:C0003315,
umls-concept:C0007634,
umls-concept:C0030685,
umls-concept:C0123759,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C0752312,
umls-concept:C1283071,
umls-concept:C1879547,
umls-concept:C1963578,
umls-concept:C2587213
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pubmed:issue |
24
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pubmed:dateCreated |
2000-1-27
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pubmed:abstractText |
Activation of antigen-presenting cells (APCs) by invariant constituents of pathogens such as lipopolysaccharide (LPS) or bacterial DNA (CpG-DNA) initiates immune responses. We have analyzed the mitogen-activated protein kinase (MAPK) pathways triggered by CpG-DNA and their significance for cytokine production in two subsets of APCs, i.e. macrophages and dendritic cells (DCs). We found that CpG-DNA induced extracellular signal-regulated kinase (ERK) activity in macrophages in a classic MEK-dependent way. This pathway up-regulated tumor necrosis factor production but down-regulated interleukin (IL)-12 production. However, in DCs, which produce large amounts of IL-12, CpG-DNA and LPS failed to induce ERK activity. Consistent with a specific negative regulatory role for ERK in macrophages, chemical activation of this pathway in DCs suppressed CpG-DNA-induced IL-12 production. Overall, these results imply that differential activation of MAP kinase pathways is a basic mechanism by which distinct subsets of innate immune cells regulate their effector functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
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pubmed:pagination |
6973-82
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10601019-Animals,
pubmed-meshheading:10601019-Antigen-Presenting Cells,
pubmed-meshheading:10601019-Bone Marrow Cells,
pubmed-meshheading:10601019-Cell Line,
pubmed-meshheading:10601019-Cells, Cultured,
pubmed-meshheading:10601019-Dendritic Cells,
pubmed-meshheading:10601019-Dinucleoside Phosphates,
pubmed-meshheading:10601019-Gene Expression Regulation,
pubmed-meshheading:10601019-Interleukin-12,
pubmed-meshheading:10601019-Lipopolysaccharides,
pubmed-meshheading:10601019-Luciferases,
pubmed-meshheading:10601019-Macrophages,
pubmed-meshheading:10601019-Mice,
pubmed-meshheading:10601019-Mice, Inbred C57BL,
pubmed-meshheading:10601019-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10601019-Oligodeoxyribonucleotides,
pubmed-meshheading:10601019-Recombinant Proteins,
pubmed-meshheading:10601019-Thionucleotides,
pubmed-meshheading:10601019-Transfection,
pubmed-meshheading:10601019-Tumor Necrosis Factor-alpha
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|
pubmed:year |
1999
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pubmed:articleTitle |
Cell type-specific activation of mitogen-activated protein kinases by CpG-DNA controls interleukin-12 release from antigen-presenting cells.
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pubmed:affiliation |
Institute of Medical Microbiology, Technische Universität München, Trogerstrasse 9, D-81675 Munich.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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