Linked Life Data

10556881

Source:http://linkedlifedata.com/resource/pubmed/id/10556881

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-4-14
pubmed:abstractText
We have used surface plasmon resonance (SPR) to follow variations in the concentrations of binary complexes as RNA polymerase moves into a transcriptionally competent initiation complex with immobilized DNA fibres containing promoter sequences. The use of SPR to follow complex binding phenomena is described. We have also followed the changes in the mass of initiation complexes following addition of the nucleotide triphosphates prerequisite for transcription on the immobilized template. These signals are interpreted in terms of the escape of RNA polymerase into elongation mode and the subsequent synthesis of nascent RNA molecules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0952-3499
pubmed:author
pubmed:copyrightInfo
Copyright 1999 John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
322-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Real time in vitro analysis of transcription by RNA polymerase on immobilized DNA fibres.
pubmed:affiliation
Unité de Physicochimie des Macromolécules Biologiques (URA 1149 du CNRS), Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris cédex 15, France.
pubmed:publicationType
Journal Article