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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1999-12-23
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pubmed:abstractText |
CTLA-4, expressed by activated T cells, transduces an inhibitory signal. We show here that PCR amplification of the coding sequence of CTLA-4 in nonstimulated human T lymphocytes results in the amplification of two transcripts of 650 and 550 bp. Sequencing shows that the larger form codes for membrane CTLA-4 and the 550-bp transcript is a spliced variant in which exon 2 coding for the transmembrane region is deleted. This spliced cDNA has been named CTLA-4delTM. The splicing induces a frame shift which results in the addition of 22 extra amino acids before a translational termination. Activation of T cells with phorbol 12-myristate 13-acetate plus ionomycin or anti-CD3 plus anti-CD28 monoclonal antibodies induces a suppression of CTLA-4delTM mRNA expression associated with a preferential expression of the membrane CTLA-4 mRNA, showing that CTLA-4delTM mRNA expression is restricted to nonactivated T cells. A soluble immunoreactive form of CTLA-4 was detected in the serum of 14 / 64 healthy subjects. These results suggest that nonstimulated T cells may constitutively produce a soluble form of CTLA-4 which may have an important role in the regulation of immune homeostasis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoconjugates,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/abatacept
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3596-602
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10556814-Alternative Splicing,
pubmed-meshheading:10556814-Amino Acid Sequence,
pubmed-meshheading:10556814-Animals,
pubmed-meshheading:10556814-Antigens, CD,
pubmed-meshheading:10556814-Antigens, Differentiation,
pubmed-meshheading:10556814-Base Sequence,
pubmed-meshheading:10556814-COS Cells,
pubmed-meshheading:10556814-CTLA-4 Antigen,
pubmed-meshheading:10556814-Cells, Cultured,
pubmed-meshheading:10556814-Gene Expression,
pubmed-meshheading:10556814-Humans,
pubmed-meshheading:10556814-Immunoconjugates,
pubmed-meshheading:10556814-Lymphocyte Activation,
pubmed-meshheading:10556814-Molecular Sequence Data,
pubmed-meshheading:10556814-RNA, Messenger,
pubmed-meshheading:10556814-Recombinant Fusion Proteins,
pubmed-meshheading:10556814-Solubility,
pubmed-meshheading:10556814-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
A soluble form of CTLA-4 generated by alternative splicing is expressed by nonstimulated human T cells.
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pubmed:affiliation |
Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France.
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pubmed:publicationType |
Journal Article
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