Source:http://linkedlifedata.com/resource/pubmed/id/10544080
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1999-11-26
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| pubmed:abstractText |
Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discovered soluble N-ethylmaleimide-sensitive factor attachment protein receptor-like protein termed human vesicle-associated membrane protein-associated protein of 33 kDa (hVAP-33). In vitro binding assay and in vivo coimmunoprecipitation studies confirmed the interaction between hVAP-33 and NS5A. Interestingly, hVAP-33 was also shown to interact with NS5B, the viral RNA-dependent RNA polymerase. NS5A and NS5B bind to different domains of hVAP-33: NS5A binds to the C-terminus, whereas NS5B binds to the N-terminus of hVAP-33. Immunofluorescent staining showed a significant colocalization of hVAP-33 with both NS5A and NS5B proteins. hVAP-33 contains a coiled-coil domain followed by a membrane-spanning domain at its C-terminus. Cell fractionation analysis revealed that hVAP-33 is predominantly associated with the ER, the Golgi complex, and the prelysosomal membrane, consistent with its potential role in intracellular membrane trafficking. These interactions provide a mechanism for membrane association of the HCV RNA replication complex and further suggest that NS5A is a part of the viral RNA replication complex.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NS-5 protein, hepatitis C virus,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Replicase,
http://linkedlifedata.com/resource/pubmed/chemical/VAPA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0042-6822
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
263
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
30-41
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10544080-Animals,
pubmed-meshheading:10544080-COS Cells,
pubmed-meshheading:10544080-Carrier Proteins,
pubmed-meshheading:10544080-Female,
pubmed-meshheading:10544080-Fluorescent Antibody Technique,
pubmed-meshheading:10544080-Gene Library,
pubmed-meshheading:10544080-Hepacivirus,
pubmed-meshheading:10544080-Humans,
pubmed-meshheading:10544080-Membrane Proteins,
pubmed-meshheading:10544080-Precipitin Tests,
pubmed-meshheading:10544080-RNA Replicase,
pubmed-meshheading:10544080-Rabbits,
pubmed-meshheading:10544080-Rats,
pubmed-meshheading:10544080-Subcellular Fractions,
pubmed-meshheading:10544080-Two-Hybrid System Techniques,
pubmed-meshheading:10544080-Vesicular Transport Proteins,
pubmed-meshheading:10544080-Viral Nonstructural Proteins,
pubmed-meshheading:10544080-Virus Replication
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| pubmed:year |
1999
|
| pubmed:articleTitle |
Hepatitis C virus RNA polymerase and NS5A complex with a SNARE-like protein.
|
| pubmed:affiliation |
Department of Molecular Microbiology and Immunology, University of Southern California School of Medicine, Los Angeles, California 90033, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|