Linked Life Data

10491646

Source:http://linkedlifedata.com/resource/pubmed/id/10491646

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-11-22
pubmed:abstractText
Matrix metalloproteinases (MMPs) are zinc-requiring enzymes that can degrade components of the extracellular matrix and that are implicated in tissue remodeling. Their role in the onset of menstruation in vivo has been proven; however, the expression and functions of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in vascular structures are poorly understood. We determined by immunocytochemistry, using characterized monoclonal antibodies, the distribution of MMPs and of their inhibitors TIMP-1 and TIMP-2 in the endometrium during the menstrual cycle. MMP-1, MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 had differing distributions and patterns of expression. In addition to the localization of MMP-9 in the epithelium and of MMP-2, MMP-3, and MMP-1 in the stromal tissue, these MMPs were detected in the vascular structures. MMP-2 (72-kDa gelatinase) and tissue inhibitors TIMP-1 and TIMP-2 were detectable in vessels throughout the cycle. In contrast, MMP-3 (stromelysin-1) was detected only in late-secretory and menstrual endometrial vessels, while MMP-9 (92-kDa gelatinase) was detected in spiral arteries during the secretory phase and in vascular structures during the midfollicular and menstrual phases. The expression of MMP-2 and MMP-9 in endometrial vessels during the proliferative and secretory periods suggests their relationship to vascular growth and angiogenesis. The pronounced expression of MMP-3 (stromelysin-1) in the vessels situated in the superficial endometrial layer during menses suggests that this metalloproteinase initiates damage in the vascular wall during menstrual breakdown. The finding of an intense expression of TIMP-1 and TIMP-2 in the vessels delineating necrotic from non-necrotic areas during menses also suggests that they could limit tissue damage, allowing regeneration of the endometrium after menses. These data indicate that, in addition to expression in epithelial cells and stromal tissue, MMPs are expressed in endometrial vascular cells in a cycle-specific pattern, consistent with regulation by steroid hormones and with specific roles in the vascular remodeling processes occurring in the endometrium during the cycle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1070-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10491646-Adult, pubmed-meshheading:10491646-Blood Vessels, pubmed-meshheading:10491646-Cells, Cultured, pubmed-meshheading:10491646-Endometrium, pubmed-meshheading:10491646-Endothelium, Vascular, pubmed-meshheading:10491646-Female, pubmed-meshheading:10491646-Humans, pubmed-meshheading:10491646-Immunohistochemistry, pubmed-meshheading:10491646-Matrix Metalloproteinase 2, pubmed-meshheading:10491646-Matrix Metalloproteinase 3, pubmed-meshheading:10491646-Matrix Metalloproteinase 9, pubmed-meshheading:10491646-Matrix Metalloproteinases, pubmed-meshheading:10491646-Middle Aged, pubmed-meshheading:10491646-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:10491646-Tissue Inhibitor of Metalloproteinase-2, pubmed-meshheading:10491646-Tissue Inhibitor of Metalloproteinases
pubmed:year
1999
pubmed:articleTitle
Expression of metalloproteinases and their inhibitors in blood vessels in human endometrium.
pubmed:affiliation
INSERM U460, CHU Xavier Bichat, 75870 Paris Cedex, France Laboratoire Cassenne-Hoechst Marion Roussel, Paris La Défense Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't