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rdf:type |
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lifeskim:mentions |
umls-concept:C0013138,
umls-concept:C0015392,
umls-concept:C0017262,
umls-concept:C0036098,
umls-concept:C0040648,
umls-concept:C0185117,
umls-concept:C0596746,
umls-concept:C0598312,
umls-concept:C0599894,
umls-concept:C0599946,
umls-concept:C1511662,
umls-concept:C1521761,
umls-concept:C1521840,
umls-concept:C2911684
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pubmed:issue |
9
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pubmed:dateCreated |
1999-9-10
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pubmed:abstractText |
The promoters of Drosophila genes encoding DNA replication-related proteins contain transcription regulatory elements consisting of an 8-bp palindromic DNA replication-related element (DRE) sequence (5'-TATCGATA). The specific DRE-binding factor (DREF), a homodimer of the polypeptide with 709 amino acid residues, is a positive trans-acting factor for transcription of DRE-containing genes. Both DRE binding and dimer formation are associated with residues 16 to 115 of the N-terminal region. We have established transgenic flies expressing the full-length DREF polypeptide or its N-terminal fragment (amino acid residues 1 to 125) under the control of the heat shock promoter, the salivary gland-specific promoter, or the eye imaginal disc-specific promoter. Heat shock induction of the N-terminal fragment during embryonic, larval, or pupal stages caused greater than 50% lethality. This lethality was overcome by coexpression of the full-length DREF. In salivary glands of the transgenic larvae expressing the N-terminal fragment, this fragment formed a homodimer and a heterodimer with the endogenous DREF. Ectopic expression of the N-terminal fragment in salivary gland cells reduced the contents of mRNAs for the 180-kDa subunit of DNA polymerase alpha and for dE2F and the extent of DNA endoreplication. Ectopic expression of the N-terminal fragment in the eye imaginal discs significantly reduced DNA replication in cells at the second mitotic wave. The lines of evidence suggest that the N-terminal fragment can impede the endogenous DREF function in a dominant negative manner and that DREF is required for normal DNA replication in both mitotic cell cycle and endo cycle.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-1907895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-1923767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-2239451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-2835286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-3128741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-6692991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7533262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7559650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7601349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7768188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7781065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7797583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7859740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7905482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7925015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7926754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-7958894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8022787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8093616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8114698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8223268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8414520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8543167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8632015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8662923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8665850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8670868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8672531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-8918795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9001253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9016631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9078366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9271122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9278447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9748283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10454549-9862973
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6020-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10454549-Animals,
pubmed-meshheading:10454549-Animals, Genetically Modified,
pubmed-meshheading:10454549-DNA Replication,
pubmed-meshheading:10454549-Drosophila,
pubmed-meshheading:10454549-Drosophila Proteins,
pubmed-meshheading:10454549-Eye,
pubmed-meshheading:10454549-Eye Abnormalities,
pubmed-meshheading:10454549-Gene Expression,
pubmed-meshheading:10454549-Gene Targeting,
pubmed-meshheading:10454549-Hot Temperature,
pubmed-meshheading:10454549-Insect Proteins,
pubmed-meshheading:10454549-Phenotype,
pubmed-meshheading:10454549-Salivary Glands,
pubmed-meshheading:10454549-Transcription Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Targeted expression of the DNA binding domain of DRE-binding factor, a Drosophila transcription factor, attenuates DNA replication of the salivary gland and eye imaginal disc.
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pubmed:affiliation |
Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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