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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-9-17
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pubmed:abstractText |
Umbilical cord blood (CB) is increasingly used for allogeneic hematopoietic stem cell transplantation. To determine whether viral antigen-specific cytotoxic T-lymphocytes (CTL) could be generated from the predominantly naive T-cell populations in CB, CB-derived mononuclear cells were stimulated with autologous Epstein-Barr virus (EBV) transformed B-lymphoblastoid cell lines over several weeks in the presence of recombinant human interleukin-2 (IL-2). By 28 days of culture, T-lymphocytes from all six CB that had been treated with IL-2 displayed EBV-specific cytotoxicity. These cells were largely CD4(+), with complete inhibition of cytotoxicity by anti-CD3 and variable inhibition by anti-HLA DR monoclonal antibodies. The EBV-specific effectors were cloned by limiting dilution, and most of the CTL clones were CD4(+). The cytotoxicity of the CB-derived CD4(+) CTL clones was inhibited by EGTA but not by anti-Fas ligand mAb, suggesting that this cytotoxicity was mediated by perforin/granzyme B. These data indicate that virus-specific CTL can be cultivated and cloned from CB, a human T-cell source that may not have prior in vivo antigenic exposure or reactivity. This finding may have applications in adoptive immunotherapy to recipients of CB transplants.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/GZMB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Granzymes,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0008-8749
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
195
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10448007-Adult,
pubmed-meshheading:10448007-Antibodies, Monoclonal,
pubmed-meshheading:10448007-Antigens, CD,
pubmed-meshheading:10448007-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10448007-Cell Line, Transformed,
pubmed-meshheading:10448007-Clone Cells,
pubmed-meshheading:10448007-Coculture Techniques,
pubmed-meshheading:10448007-Cytotoxicity, Immunologic,
pubmed-meshheading:10448007-Egtazic Acid,
pubmed-meshheading:10448007-Fas Ligand Protein,
pubmed-meshheading:10448007-Fetal Blood,
pubmed-meshheading:10448007-Granzymes,
pubmed-meshheading:10448007-HLA-DR Antigens,
pubmed-meshheading:10448007-Herpesvirus 4, Human,
pubmed-meshheading:10448007-Humans,
pubmed-meshheading:10448007-Immunotherapy, Adoptive,
pubmed-meshheading:10448007-Infant, Newborn,
pubmed-meshheading:10448007-Interleukin-2,
pubmed-meshheading:10448007-Membrane Glycoproteins,
pubmed-meshheading:10448007-Serine Endopeptidases,
pubmed-meshheading:10448007-T-Lymphocytes, Cytotoxic
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pubmed:year |
1999
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pubmed:articleTitle |
CD4(+) Epstein-Barr virus-specific cytotoxic T-lymphocytes from human umbilical cord blood.
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pubmed:affiliation |
The Bone Marrow Transplantation Program, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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