| pubmed-article:10438658 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0002241 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0441472 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C1511636 | lld:lifeskim |
| pubmed-article:10438658 | lifeskim:mentions | umls-concept:C0600334 | lld:lifeskim |
| pubmed-article:10438658 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10438658 | pubmed:dateCreated | 1999-8-26 | lld:pubmed |
| pubmed-article:10438658 | pubmed:abstractText | Tumor necrosis factor-alpha is assumed to play a role in toxic liver damage. We examined whether exogenous tumor necrosis factor-alpha must be present for alpha-amanitin cytotoxicity in rat hepatocyte culture. alpha-Amanitin at a concentration of 0.1 microM, which is close to that found in intoxicated patients, inhibits RNA and protein synthesis within 12 h but cytotoxicity only occurs after a latency period and is pronounced at 36 h after the start of treatment. Tumor necrosis factor-alpha is not indispensable for the development of cytotoxicity but aggravates it and leads to a time shift towards earlier times. Lipid peroxidation is low with alpha-amanitin alone even at 36 h but markedly increased by cotreatment with tumor necrosis factor-alpha. The antioxidant silibin prevents the effect of tumor necrosis factor-alpha, indicating an involvement of reactive oxygen species. alpha-Amanitin alone does not increase but dose-dependently inhibits the expression of the antioxidant enzyme manganous superoxide dismutase and decreases the inducing effect of TNF-alpha on the expression of this enzyme. The gene expression of endogenous tumor necrosis factor-alpha in the hepatocytes is not increased but rather inhibited by alpha-amanitin treatment. The results suggest that alpha-amanitin causes delayed cytotoxicity following rapid inhibition of RNA and protein synthesis and that tumor necrosis factor-alpha shortens the latency period and aggravates the cytotoxicity by a mechanism which may involve reactive oxygen species. | lld:pubmed |
| pubmed-article:10438658 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10438658 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10438658 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10438658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10438658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10438658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10438658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10438658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10438658 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10438658 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10438658 | pubmed:issn | 0041-008X | lld:pubmed |
| pubmed-article:10438658 | pubmed:author | pubmed-author:KahnTT | lld:pubmed |
| pubmed-article:10438658 | pubmed:author | pubmed-author:GerberEE | lld:pubmed |
| pubmed-article:10438658 | pubmed:author | pubmed-author:RöhrdanzEE | lld:pubmed |
| pubmed-article:10438658 | pubmed:author | pubmed-author:HorbachMM | lld:pubmed |
| pubmed-article:10438658 | pubmed:author | pubmed-author:Tran-ThiQ HQH | lld:pubmed |
| pubmed-article:10438658 | pubmed:author | pubmed-author:El-BahayCC | lld:pubmed |
| pubmed-article:10438658 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10438658 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10438658 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:10438658 | pubmed:volume | 158 | lld:pubmed |
| pubmed-article:10438658 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10438658 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10438658 | pubmed:pagination | 253-60 | lld:pubmed |
| pubmed-article:10438658 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
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| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:meshHeading | pubmed-meshheading:10438658... | lld:pubmed |
| pubmed-article:10438658 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10438658 | pubmed:articleTitle | Influence of tumor necrosis factor-alpha and silibin on the cytotoxic action of alpha-amanitin in rat hepatocyte culture. | lld:pubmed |
| pubmed-article:10438658 | pubmed:affiliation | Institute of Toxicology, University of Düsseldorf, Düsseldorf, Germany. | lld:pubmed |
| pubmed-article:10438658 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10438658 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
