Linked Life Data

10434927

Source:http://linkedlifedata.com/resource/pubmed/id/10434927

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-8-12
pubmed:abstractText
The presence of metalloproteinase activity in endometrial flushings obtained from premenopausal women, during the proliferative and secretory phases of the menstrual cycle, control post-menopausal women and women with post-menopausal bleeding (PMB) with or without adenocarcinoma was analysed by zymography. In addition, quantitative measurements of matrix metalloproteinase 2 (MMP-2), MMP-3, MMP-9 and tissue inhibitor of metalloproteinase 1 (TIMP-1) in the flushings were obtained by ELISA. The zymography results showed eight bands of activity, with molecular weights ranging from 51 to 208 kDa in the flushings from pre-menopausal women and post-menopausal women, particularly those with adenocarcinoma. Both zymography and ELISA showed that MMP-2 and MMP-9 were the major metalloproteinases found in the flushings and only low concentrations of MMP-3 were found. Concentrations of MMP-2 in pre-menopausal women were higher in flushings obtained during the secretory phase of the menstrual cycle than those obtained in the proliferative phase (P < 0.05), suggesting that it may play a role in embryo implantation. Concentrations of MMP-2 (P < 0.001), MMP-9 (P < 0.05) and TIMP-1 (P < 0.001) in the flushings from post-menopausal control women were lower than those from pre-menopausal women. Concentrations of MMP-2 (P < 0.05) and TIMP-1 (P < 0.05) were higher in flushings from women with PMB without carcinoma compared with post-menopausal controls and concentrations of MMP-9 (P < 0.01) and TIMP-1 (P < 0.05) in flushings from women with adenocarcinoma were higher than in post-menopausal controls. Among subjects with PMB, concentrations of MMP-9 in women with adenocarcinoma were higher than those without carcinoma (P < 0.05). Our results show that concentrations of MMP-2, MMP-9 and TIMP-1, but not MMP-3, are associated with endometrial activity and, therefore, may have a role in the breakdown of endometrial tissue. In addition, the increased concentrations of MMP-9 in flushings of women with adenocarcinoma indicate that this particular proteinase is associated with the presence of endometrial neoplastic cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10434927-Adenocarcinoma, pubmed-meshheading:10434927-Adult, pubmed-meshheading:10434927-Aged, pubmed-meshheading:10434927-Aged, 80 and over, pubmed-meshheading:10434927-Collagenases, pubmed-meshheading:10434927-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:10434927-Endometrial Neoplasms, pubmed-meshheading:10434927-Endometrium, pubmed-meshheading:10434927-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:10434927-Female, pubmed-meshheading:10434927-Follicular Phase, pubmed-meshheading:10434927-Gelatinases, pubmed-meshheading:10434927-Humans, pubmed-meshheading:10434927-Luteal Phase, pubmed-meshheading:10434927-Matrix Metalloproteinase 2, pubmed-meshheading:10434927-Matrix Metalloproteinase 3, pubmed-meshheading:10434927-Matrix Metalloproteinase 9, pubmed-meshheading:10434927-Menopause, pubmed-meshheading:10434927-Metalloendopeptidases, pubmed-meshheading:10434927-Middle Aged, pubmed-meshheading:10434927-Statistics, Nonparametric, pubmed-meshheading:10434927-Tissue Inhibitor of Metalloproteinase-1
pubmed:year
1999
pubmed:articleTitle
Metalloproteinases and tissue inhibitor of metalloproteinase 1 (TIMP-1) in endometrial flushings from pre- and post-menopausal women and from women with endometrial adenocarcinoma.
pubmed:affiliation
Biomedical Research Centre, Sheffield Hallam University, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't