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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0008643,
umls-concept:C0008654,
umls-concept:C0014442,
umls-concept:C0017337,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0450429,
umls-concept:C0723595,
umls-concept:C0796679,
umls-concept:C1412186,
umls-concept:C1456820,
umls-concept:C1521193,
umls-concept:C1880022
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pubmed:issue |
8
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pubmed:dateCreated |
1999-9-21
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pubmed:abstractText |
Numerous proteins are cleaved or "shed" from their membrane-bound form. One such protein, tumour necrosis factor alpha (TNF-alpha), is synthesized as a type 2 transmembrane protein. Recently, a human protease responsible for this shedding, the TNF-alpha converting enzyme (TACE/ADAM17), was isolated. TACE/ADAM17 is a member of the adamalysin class of zinc-binding metalloproteases or ADAM (a disintegrin and metalloprotease). We report the isolation and characterization of the mouse TACE/ADAM17 cDNA and gene. Mouse TACE/ADAM17 has a 92% amino-acid identity with the human protein and was ubiquitously expressed. A recombinant form of the protease is found to cleave a peptide representing the cleavage site of precursor mouse TNF-alpha. An alternatively spliced form of mouse TACE/ADAM17 was found that would produce a soluble protein. The gene for TACE/ADAM17 is approximately 50 kb and contains 19 exons. Chromosomal mapping places TACE/ADAM17 on mouse chromosome 12 and human chromosome 2p25.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
541-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10433800-ADAM Proteins,
pubmed-meshheading:10433800-Amino Acid Sequence,
pubmed-meshheading:10433800-Animals,
pubmed-meshheading:10433800-Base Sequence,
pubmed-meshheading:10433800-Chromosome Mapping,
pubmed-meshheading:10433800-Chromosomes, Human, Pair 2,
pubmed-meshheading:10433800-DNA, Complementary,
pubmed-meshheading:10433800-Exons,
pubmed-meshheading:10433800-Genomic Library,
pubmed-meshheading:10433800-Humans,
pubmed-meshheading:10433800-Introns,
pubmed-meshheading:10433800-Metalloendopeptidases,
pubmed-meshheading:10433800-Mice,
pubmed-meshheading:10433800-Molecular Sequence Data,
pubmed-meshheading:10433800-Recombinant Proteins,
pubmed-meshheading:10433800-Restriction Mapping,
pubmed-meshheading:10433800-Sequence Alignment,
pubmed-meshheading:10433800-Sequence Homology, Amino Acid,
pubmed-meshheading:10433800-Tumor Necrosis Factor-alpha
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pubmed:year |
1999
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pubmed:articleTitle |
Characterization of the cDNA and gene for mouse tumour necrosis factor alpha converting enzyme (TACE/ADAM17) and its location to mouse chromosome 12 and human chromosome 2p25.
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pubmed:affiliation |
Immunex Corporation, 51 University St., Seattle, WA, 98101, USA. cerretti@immunex.com
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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