Source:http://linkedlifedata.com/resource/pubmed/id/10432221
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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0040300,
umls-concept:C0185117,
umls-concept:C0205314,
umls-concept:C0529099,
umls-concept:C0597298,
umls-concept:C0679622,
umls-concept:C0748342,
umls-concept:C1553877,
umls-concept:C1608885,
umls-concept:C1880274,
umls-concept:C2911684
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pubmed:issue |
1-2
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pubmed:dateCreated |
1999-9-8
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pubmed:databankReference | |
pubmed:abstractText |
cDNAs coding for bovine estrogen receptor beta (ERbeta) isoforms were cloned from bovine granulosa cells using a combination of several RT-PCR strategies. The cloned full-length receptor contains an open reading frame of 474 amino acids encoding a protein with high homology to the ERbeta sequences from other species. A second isoform nearly totally lacking the ligand binding domain was cloned that is expressed to relatively high levels in reproductive tissues. Expression of both ERbeta isoforms is down-regulated in corpus luteum and endometrium during the luteal phase of the female cycle. In addition, in granulosa cells several ERbeta isoforms carrying major internal deletions were detected by RT-PCR and cloned. Transient transfection studies expressing the two major bovine ERbeta isoforms together with an ERE reporter construct show estrogen-dependent transactivation by the full-length isoform, whereas the isoform lacking the ligand binding domain did not show any transactivation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0303-7207
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-45
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10432221-Amino Acid Sequence,
pubmed-meshheading:10432221-Animals,
pubmed-meshheading:10432221-Base Sequence,
pubmed-meshheading:10432221-Cattle,
pubmed-meshheading:10432221-Cloning, Molecular,
pubmed-meshheading:10432221-DNA, Complementary,
pubmed-meshheading:10432221-Estrogen Receptor beta,
pubmed-meshheading:10432221-Female,
pubmed-meshheading:10432221-Granulosa Cells,
pubmed-meshheading:10432221-Molecular Sequence Data,
pubmed-meshheading:10432221-Protein Isoforms,
pubmed-meshheading:10432221-Receptors, Estrogen
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pubmed:year |
1999
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pubmed:articleTitle |
Cloning of bovine estrogen receptor beta (ERbeta): expression of novel deleted isoforms in reproductive tissues.
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pubmed:affiliation |
Institute for Hormone and Fertility Research, University of Hamburg, Germany. walther@ihf.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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