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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1999-8-16
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pubmed:abstractText |
An important aspect of the specification of neuronal fate is the choice of neurotransmitter. In Caenorhabditis elegans the neurotransmitter GABA is synthesized by the UNC-25 glutamic acid decarboxylase (GAD) and packaged into synaptic vesicles by the UNC-47 transporter. Both unc-25 and unc-47 are expressed in 26 GABAergic neurons of five different types. Previously, we have identified that the unc-30 homeobox gene controls the fate of 19 type D GABAergic neurons. We report here that the UNC-30 homeodomain protein transcriptionally regulates the expression of unc-25 and unc-47 in the 19 type D neurons. UNC-30 bound to the unc-25 and unc-47 promoters sequence-specifically. Mutations in the UNC-30 binding sites of the unc-25 and unc-47 promoters abolished the expression of reporter genes in the D neurons. The ectopic expression of UNC-30 induced the ectopic expression of reporter genes driven by the wild-type unc-25 and unc-47 promoters. Our data establish a mechanism for cell type-specific transcriptional coregulation of genes required for the synthesis and packaging of the neurotransmitter GABA.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GABA Plasma Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Anion Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Inhibitory Amino Acid...,
http://linkedlifedata.com/resource/pubmed/chemical/unc-30 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/unc-47 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6225-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10414952-Animals,
pubmed-meshheading:10414952-Base Sequence,
pubmed-meshheading:10414952-Caenorhabditis elegans,
pubmed-meshheading:10414952-Caenorhabditis elegans Proteins,
pubmed-meshheading:10414952-Carrier Proteins,
pubmed-meshheading:10414952-DNA Footprinting,
pubmed-meshheading:10414952-GABA Plasma Membrane Transport Proteins,
pubmed-meshheading:10414952-Glutamate Decarboxylase,
pubmed-meshheading:10414952-Green Fluorescent Proteins,
pubmed-meshheading:10414952-Helminth Proteins,
pubmed-meshheading:10414952-Homeodomain Proteins,
pubmed-meshheading:10414952-Luminescent Proteins,
pubmed-meshheading:10414952-Male,
pubmed-meshheading:10414952-Membrane Proteins,
pubmed-meshheading:10414952-Membrane Transport Proteins,
pubmed-meshheading:10414952-Molecular Sequence Data,
pubmed-meshheading:10414952-Mutation,
pubmed-meshheading:10414952-Neurons,
pubmed-meshheading:10414952-Nuclear Proteins,
pubmed-meshheading:10414952-Organic Anion Transporters,
pubmed-meshheading:10414952-Promoter Regions, Genetic,
pubmed-meshheading:10414952-Transcription, Genetic,
pubmed-meshheading:10414952-Vesicular Inhibitory Amino Acid Transport Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
Coordinated transcriptional regulation of the unc-25 glutamic acid decarboxylase and the unc-47 GABA vesicular transporter by the Caenorhabditis elegans UNC-30 homeodomain protein.
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pubmed:affiliation |
Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz, California 95064, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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