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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-9-3
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pubmed:abstractText |
We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol-DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol-DOPE liposomes and DC-Chol-DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol-DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
1419
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
186-94
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10407070-Cell Line, Transformed,
pubmed-meshheading:10407070-Cholesterol,
pubmed-meshheading:10407070-DNA,
pubmed-meshheading:10407070-Epithelial Cells,
pubmed-meshheading:10407070-Fluorescent Dyes,
pubmed-meshheading:10407070-Gene Therapy,
pubmed-meshheading:10407070-Humans,
pubmed-meshheading:10407070-Liposomes,
pubmed-meshheading:10407070-Microscopy, Confocal,
pubmed-meshheading:10407070-Microscopy, Electron,
pubmed-meshheading:10407070-Organic Chemicals,
pubmed-meshheading:10407070-Phosphatidylethanolamines,
pubmed-meshheading:10407070-Trachea,
pubmed-meshheading:10407070-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Gene transfection efficiency of tracheal epithelial cells by DC-chol-DOPE/DNA complexes.
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pubmed:affiliation |
Dipartimento di Biopatologia e Diagnostica per Immagini, Università di Roma 'Tor Vergata' and Istituto CSS-Mendel, Roma, Via di Tor Vergata 135, 00133, Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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