Source:http://linkedlifedata.com/resource/pubmed/id/10382739
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
1999-7-8
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| pubmed:abstractText |
Adjuvants that can improve mucosal vaccine efficacy are much warranted. In this comparative study between cholera toxin (CT) and immune-stimulating complexes (ISCOM) we found that, contrary to CT, ovalbumin (OVA)-ISCOM were poor inducers of mucosal anti-OVA IgA responses, but induced similar or better systemic immunity following oral immunizations. The addition of CT to the oral OVA-ISCOM protocol did not stimulate local anti-OVA IgA immunity, nor did it change the quality or magnitude of the systemic responses. Both vectors recruited strong innate immunity, but only OVA-ISCOM could directly induce IL-12, demonstrable at the protein and mRNA levels. CT had no inhibitory effects on lipopolysaccharide/IFN-gamma-induced IL-12 mRNA expression or IL-12 production. Furthermore, adjuvanticity of CT was unaffected in IL-12-deficient mice, while OVA-ISCOM showed partly impaired adjuvant effects by the lack of IL-12. CT abrogated the induction of oral tolerance stimulated by antigen feeding in these mice. In addition, CT did not alter TGF-beta levels, suggesting that the immunomodulating effect of CT was independent of IL-12 as well as TGF-beta production. Taken together, these findings indicate that mucosal adjuvanticity of CT and ISCOM are differently dependent on IL-12, suggesting that separate and distinct antigen-processing pathways are involved.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/ISCOMs,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
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| pubmed:issn |
0014-2980
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1774-84
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10382739-Adjuvants, Immunologic,
pubmed-meshheading:10382739-Administration, Oral,
pubmed-meshheading:10382739-Animals,
pubmed-meshheading:10382739-Base Sequence,
pubmed-meshheading:10382739-Cholera Toxin,
pubmed-meshheading:10382739-DNA Primers,
pubmed-meshheading:10382739-ISCOMs,
pubmed-meshheading:10382739-Immune Tolerance,
pubmed-meshheading:10382739-Immunity, Mucosal,
pubmed-meshheading:10382739-Immunoglobulin G,
pubmed-meshheading:10382739-Interleukin-12,
pubmed-meshheading:10382739-Lymphocyte Activation,
pubmed-meshheading:10382739-Mice,
pubmed-meshheading:10382739-Mice, Inbred BALB C,
pubmed-meshheading:10382739-Mice, Inbred C57BL,
pubmed-meshheading:10382739-Mice, Knockout,
pubmed-meshheading:10382739-Ovalbumin,
pubmed-meshheading:10382739-RNA, Messenger,
pubmed-meshheading:10382739-T-Lymphocytes
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| pubmed:year |
1999
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| pubmed:articleTitle |
The mucosal adjuvant effects of cholera toxin and immune-stimulating complexes differ in their requirement for IL-12, indicating different pathways of action.
|
| pubmed:affiliation |
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|