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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5421
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pubmed:dateCreated |
1999-6-25
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pubmed:abstractText |
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1811-6
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:10364548-Animals,
pubmed-meshheading:10364548-Cells, Cultured,
pubmed-meshheading:10364548-Dendrites,
pubmed-meshheading:10364548-Electric Stimulation,
pubmed-meshheading:10364548-Hippocampus,
pubmed-meshheading:10364548-Humans,
pubmed-meshheading:10364548-Long-Term Potentiation,
pubmed-meshheading:10364548-Neuronal Plasticity,
pubmed-meshheading:10364548-Neurons,
pubmed-meshheading:10364548-Organ Culture Techniques,
pubmed-meshheading:10364548-Rats,
pubmed-meshheading:10364548-Receptor Aggregation,
pubmed-meshheading:10364548-Receptors, AMPA,
pubmed-meshheading:10364548-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:10364548-Recombinant Fusion Proteins,
pubmed-meshheading:10364548-Synapses,
pubmed-meshheading:10364548-Synaptic Transmission,
pubmed-meshheading:10364548-Tetany
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pubmed:year |
1999
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pubmed:articleTitle |
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
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pubmed:affiliation |
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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