Linked Life Data

10353324

Source:http://linkedlifedata.com/resource/pubmed/id/10353324

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-7-22
pubmed:abstractText
The metabolism of 4-(14)C-d-aldosterone (at 3 nM) was studied in the primary target organ of the hormone, in renal cortical and medullary cell cultures obtained from Wistar rats. Larger amounts of aldosterone were metabolized in medullary cells than in cortical cells, as measured by a decreased 4-(14)C-d-aldosterone radioactivity concentration (26+/-9% and 12+/-7% of the initial aldosterone added, respectively (n=5, p<0.05)). The 14C radiometabolites of aldosterone in both cultures co-chromatographed with 5alpha dihydro- (DHA) and 3alpha,3beta tetrahydroaldosterone (THA). Aldosterone metabolism was totally inhibited by a mineralocorticoid receptor antagonist canrenoat (Soldactone) (at 10(-5) to 10(-3) M), while the glucocorticoid receptor antagonist RU 38486 (Roussel UCLAF) (at 10(-5) to 10(-4) M) had no effect. Thus, the study confirmed that, in rat kidney, aldosterone can be converted to its reduced metabolites by a metabolism which is inhibited by a mineralocorticoid receptor antagonist. This indicated that the metabolism might play some role in modulation of the intracellular response to aldosterone in the kidney.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0036-5513
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Aldosterone metabolism in cultures of rat renal cortical and medullary cells.
pubmed:affiliation
Medical Department P, Rigshospitalet, Copenhagen, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't