Linked Life Data

10338524

Source:http://linkedlifedata.com/resource/pubmed/id/10338524

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-6-28
pubmed:abstractText
There is an epidemiological association between influenza virus infection and meningococcal disease. Proposed mechanisms are the destruction of the normal epithelial barrier function of the upper respiratory tract by influenza virus or the expression of human or viral surface-exposed proteins that enhance bacterial adherence and/or invasion. To test these hypotheses, human nasopharyngeal mucosa specimens from a total of 19 individual donors were successfully infected with influenza B virus and then inoculated with serogroup B Neisseria meningitidis. Subsequent bacterial association with the epithelial surface was measured in three separate series of experiments by using transmission electron microscopy (n = 6), scanning electron microscopy (n = 6), and counting of viable bacteria within homogenates of explants (n = 7). Penetration of the mucosa was estimated by measuring the count of viable bacteria recovered from explants after exposure to sodium taurocholate. Bacterial association with the surface of explants was time dependent over 24 h of superinfection. Influenza virus did not positively or negatively influence bacterial attachment to or penetration of explant mucosa compared to those of uninfected controls, even when the period of preincubation with virus was extended to 7 days. When proteins were purified from mucosal epithelium and immobilized on nitrocellulose membranes, N. meningitidis attached predominantly to bands corresponding to proteins of 210 and 130 kDa. In the presence of influenza virus infection, these proteins were gradually lost over the course of 72 h. In conclusion, influenza B virus did not increase association of serogroup B N. meningitidis with human nasopharyngeal mucosa.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-1678811, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-2025124, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-2117679, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-3123263, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-3510261, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-4293362, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-6166842, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-6413594, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-6875288, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-7130749, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-7706733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-7752215, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-7822008, http://linkedlifedata.com/resource/pubmed/commentcorrection/10338524-8757826
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3082-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Coinfection with influenza B virus does not affect association of Neisseria meningitidis with human nasopharyngeal mucosa in organ culture.
pubmed:affiliation
Division of Molecular and Genetic Medicine, F Floor, and Division of Surgical and Anaesthetic Sciences, University of Sheffield Medical School, Sheffield S10 2RX, United Kingdom. r.c.read@sheffield.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't