Linked Life Data

10330910

Source:http://linkedlifedata.com/resource/pubmed/id/10330910

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1999-6-21
pubmed:abstractText
A microfabricated injection valve incorporating a porous membrane structure is reported that enables electrokinetic concentration of DNA samples using homogeneous buffer conditions followed by injection into a channel for electrophoretic analysis. The porous membrane was incorporated in the microchannel manifold by having two channels separated from each other by 3-12 microns and connected by a thin porous silicate layer. This design allows the passage of current to establish an electrical connection between the separated channels but prevents large molecules, e.g., DNA, from traversing the membrane. Concentrated DNA can be injected into the separation channel and electrophoretically analyzed. Experiments exhibit a nonlinear increase in concentration with time, and DNA fragments can be concentrated up to 2 orders of magnitude as shown by comparison of peak intensities for analysis performed with and without concentration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1815-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Microfabricated porous membrane structure for sample concentration and electrophoretic analysis.
pubmed:affiliation
Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Tennessee 37831-6142, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't