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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-6-1
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pubmed:abstractText |
Hemodynamic forces play a key role in the modulation of the morphology and function of the endothelium by activating several kinases. We have previously shown that cyclic strain, a repetitive mechanical stretch, induces activation of extracellular signal-regulated protein kinases 1 and 2 (ERK1/2), members of the mitogen activated protein (MAP) kinase family. In order to investigate the upstream pathway of strain-induced ERK1/2 activation, we examined p21ras activation by cyclic strain and the effect of wortmannin and LY294002, phosphatidylinositol-3 kinase (PI 3-kinase) inhibitors on ERK1/2 phosphorylation. Cyclic strain induced a transient and rapid activation of p21ras at 1 min after strain. Wortmannin inhibited strain-induced ERK1/2 activation by 56.3 and 86.3 %, respectively. LY294002 inhibited ERK1 activation completely and ERK2 activation by 42.9%. These results suggest a possible involvement of p21ras and PI 3-kinase in the signal transduction pathway leading to the strain-induced ERK1/2 activation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
668-71
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10208841-Androstadienes,
pubmed-meshheading:10208841-Animals,
pubmed-meshheading:10208841-Aorta,
pubmed-meshheading:10208841-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:10208841-Cattle,
pubmed-meshheading:10208841-Cells, Cultured,
pubmed-meshheading:10208841-Chromones,
pubmed-meshheading:10208841-Endothelium, Vascular,
pubmed-meshheading:10208841-Enzyme Activation,
pubmed-meshheading:10208841-Guanosine Triphosphate,
pubmed-meshheading:10208841-Hemodynamics,
pubmed-meshheading:10208841-Morpholines,
pubmed-meshheading:10208841-Oncogene Protein p21(ras),
pubmed-meshheading:10208841-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:10208841-Phosphorylation,
pubmed-meshheading:10208841-Signal Transduction,
pubmed-meshheading:10208841-Stress, Mechanical,
pubmed-meshheading:10208841-Time Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Phosphatidylinositol-3 kinase dependent MAP kinase activation via p21ras in endothelial cells exposed to cyclic strain.
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pubmed:affiliation |
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut, 06510, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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