Source:http://linkedlifedata.com/resource/pubmed/id/10198164
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1999-5-6
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pubmed:abstractText |
The PKHD1 (polycystic kidney and hepatic disease 1) gene responsible for autosomal recessive polycystic kidney disease has been mapped to 6p21.1-p12 to an approximately 1-cM interval flanked by the markers D6S1714/D6S243 and D6S1024. We have developed a sequence-ready BAC/PAC-based contig map of this region as the next step for the positional cloning of PKHD1. This contig comprising 52 clones spanning approximately 1 Mb was established by content mapping of 44 BAC/PAC-end-derived STSs, 3 known genetic markers, 5 YAC-end-derived STSs, 3 random STSs, 1 previously mapped gene, and 1 EST. The average depth per marker is 6.3 clones, and the average STS density is 20 kb. The genomic clone overlaps were confirmed by restriction fragment fingerprint analysis. A high-resolution BAC/PAC-based contig map is essential to the ultimate goal of identifying the PKHD1 gene.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0888-7543
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pubmed:author |
pubmed-author:AvnerE DED,
pubmed-author:DixitM PMP,
pubmed-author:GerminoG GGG,
pubmed-author:GoncharukA NAN,
pubmed-author:Guay-WoodfordLL,
pubmed-author:HayashiTT,
pubmed-author:KneitzSS,
pubmed-author:OnuchicL FLF,
pubmed-author:ParkJ HJH,
pubmed-author:SantarinaL BLB,
pubmed-author:SomloSS,
pubmed-author:WuGG,
pubmed-author:ZerresKK
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
249-55
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10198164-Chromosomes, Human, Pair 6,
pubmed-meshheading:10198164-Contig Mapping,
pubmed-meshheading:10198164-DNA,
pubmed-meshheading:10198164-DNA Fingerprinting,
pubmed-meshheading:10198164-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:10198164-Humans,
pubmed-meshheading:10198164-Polycystic Kidney, Autosomal Recessive,
pubmed-meshheading:10198164-Sequence Tagged Sites
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pubmed:year |
1999
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pubmed:articleTitle |
A 1-Mb BAC/PAC-based physical map of the autosomal recessive polycystic kidney disease gene (PKHD1) region on chromosome 6.
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pubmed:affiliation |
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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