Source:http://linkedlifedata.com/resource/pubmed/id/10096854
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1999-6-7
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pubmed:abstractText |
Flavonoids isolated from citrus were evaluated for their ability to affect the inflammation response through suppression of cytokine expression by human monocytes. Several polymethoxylated flavones inhibited lipopolysaccharide-induced monocyte expression of tumor necrosis factor (TNFalpha). Subsequent studies centered on the compound 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF) which produced the highest inhibition (IC50 = 5 microM). HMF was also a potent inhibitor of macrophage inflammatory protein-1alpha (MIP-1alpha) and interleukin-10 (IL-10) production, but not of IL-1beta, IL-6, or IL-8 production. Suppression of TNFalpha production was at the level of mRNA induction as determined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). HMF was also a potent inhibitor of human phosphodiesterase activity and was shown to induce a substantial elevation of cAMP levels in monocytes. The similarity of these results to the inhibition profile of the known phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine, suggests that the polymethoxylated flavones inhibit cytokine production in part by suppression of phosphodiesterase activity. The ability of HMF to also inhibit IL-10 production suggests the additional existence of a phosphodiesterase-independent mechanism for this compound.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3,3',4',5,6,7,8-heptamethoxyflavone,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0163-3864
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
441-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10096854-Citrus,
pubmed-meshheading:10096854-Cyclic AMP,
pubmed-meshheading:10096854-Flavonoids,
pubmed-meshheading:10096854-Humans,
pubmed-meshheading:10096854-Lipopolysaccharides,
pubmed-meshheading:10096854-Monocytes,
pubmed-meshheading:10096854-Phosphodiesterase Inhibitors,
pubmed-meshheading:10096854-RNA, Messenger,
pubmed-meshheading:10096854-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10096854-Tumor Necrosis Factor-alpha
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pubmed:year |
1999
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pubmed:articleTitle |
Polymethoxylated flavones derived from citrus suppress tumor necrosis factor-alpha expression by human monocytes.
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pubmed:affiliation |
USDA, Agricultural Research Service, SAA, U.S. Citrus and Subtropical Products Laboratory, 600 Avenue S, NW, Winter Haven, Florida 33881, USA. jamwh@juno.com
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pubmed:publicationType |
Journal Article,
In Vitro
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