Source:http://linkedlifedata.com/resource/pubmed/id/10029277
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1999-7-20
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| pubmed:abstractText |
Tissue accumulation of polymorphonuclear neutrophils (PMN) in Inflammatory Bowel disease (IBD) might be, in part, due to a delay in apoptotic processes associated with the effects of their specific growth factors and inflammatory cytokines. We addressed this hypothesis by examining the activity of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage CSF (GM-CSF) in the organ culture supernatants of colonic mucosal specimens and their regulatory effects on PMN apoptosis in patients with IBD. The contents of G-CSF and GM-CSF in the supernatants were measured by the enzyme-linked immunosorbent assays and PMN apoptosis was evaluated by acridine orange/ethidium bromide staining, respectively. Mucosal specimens obtained from patients with active IBD exhibited higher levels of G-CSF and GM-CSF activity than controls. Notably, the levels of G-CSF activity were approximately 1000-fold higher than those of GM-CSF activity. Freshly isolated PMN showed a time-related increase in the proportion of cells with characteristic features of apoptosis when they were incubated with the culture medium alone and exposure of PMN to recombinant G-CSF and GM-CSF caused a concentration-dependent inhibition of apoptosis. Incubation of PMN with the supernatants from patients with active IBD induced an inhibitory effect on PMN apoptosis; this effect was abrogated to a significant degree by pre-incubation of the supernatants with anti-G-CSF serum. This study suggests that PMN apoptosis may be delayed under the influence of soluble mediators, especially G-CSF, in the microenvironment of IBD-affected mucosa, thus providing possible mechanisms for tissue accumulation of PMN in IBD.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acridine Orange,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Ethidium,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
0815-9319
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
46-53
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| pubmed:dateRevised |
2009-11-3
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| pubmed:meshHeading |
pubmed-meshheading:10029277-Acridine Orange,
pubmed-meshheading:10029277-Adolescent,
pubmed-meshheading:10029277-Adult,
pubmed-meshheading:10029277-Aged,
pubmed-meshheading:10029277-Aged, 80 and over,
pubmed-meshheading:10029277-Apoptosis,
pubmed-meshheading:10029277-Culture Media, Conditioned,
pubmed-meshheading:10029277-Culture Techniques,
pubmed-meshheading:10029277-Cytokines,
pubmed-meshheading:10029277-Ethidium,
pubmed-meshheading:10029277-Female,
pubmed-meshheading:10029277-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:10029277-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:10029277-Humans,
pubmed-meshheading:10029277-Inflammatory Bowel Diseases,
pubmed-meshheading:10029277-Intestinal Mucosa,
pubmed-meshheading:10029277-Male,
pubmed-meshheading:10029277-Middle Aged,
pubmed-meshheading:10029277-Neutrophils
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| pubmed:year |
1999
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| pubmed:articleTitle |
Increased mucosal production of granulocyte colony-stimulating factor is related to a delay in neutrophil apoptosis in Inflammatory Bowel disease.
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| pubmed:affiliation |
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
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| pubmed:publicationType |
Journal Article,
In Vitro
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