Linked Life Data

10024343

Source:http://linkedlifedata.com/resource/pubmed/id/10024343

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-4-20
pubmed:abstractText
According to the current theory of synaptic transmission, the amplitude of evoked synaptic potentials correlates with the number of synaptic vesicles released at the presynaptic terminals. Synaptic vesicles in presynaptic boutons constitute two distinct pools, namely, exo/endo cycling and reserve pools (). We defined the vesicles that were endocytosed and exocytosed during high K+ stimulation as the exo/endo cycling vesicle pool. To determine the role of exo/endo cycling vesicle pool in synaptic transmission, we estimated the quantal content electrophysiologically, whereas the pool size was determined optically using fluorescent dye FM1-43. We then manipulated the size of the pool with following treatments. First, to change the state of boutons of nerve terminals, motoneuronal axons were severed. With this treatment, the size of exo/endo cycling vesicle pool decreased together with the quantal content. Second, we promoted the FM1-43 uptake using cyclosporin A, which inhibits calcineurin activities and enhances endocytosis. Cyclosporin A increased the total uptake of FM1-43, but neither the size of exo/endo cycling vesicle pool nor the quantal content changed. Third, we increased the size of exo/endo cycling vesicle pool by forskolin, which enhances synaptic transmission. The forskolin treatment increased both the size of exo/endo cycling vesicle pool and the quantal content. Thus, we found that the quantal content was closely correlated with the size of exo/endo cycling vesicle pool but not necessarily with the total uptake of FM1-43 fluorescence by boutons. The results suggest that vesicles in the exo/endo cycling pool primarily participate in evoked exocytosis of vesicles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1557-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10024343-Animals, pubmed-meshheading:10024343-Axotomy, pubmed-meshheading:10024343-Bucladesine, pubmed-meshheading:10024343-Cyclosporine, pubmed-meshheading:10024343-Cytochalasins, pubmed-meshheading:10024343-Drosophila melanogaster, pubmed-meshheading:10024343-Endocytosis, pubmed-meshheading:10024343-Enzyme Inhibitors, pubmed-meshheading:10024343-Evoked Potentials, pubmed-meshheading:10024343-Exocytosis, pubmed-meshheading:10024343-Fluorescent Dyes, pubmed-meshheading:10024343-Forskolin, pubmed-meshheading:10024343-Larva, pubmed-meshheading:10024343-Mutation, pubmed-meshheading:10024343-Neuromuscular Junction, pubmed-meshheading:10024343-Potassium, pubmed-meshheading:10024343-Presynaptic Terminals, pubmed-meshheading:10024343-Pyridinium Compounds, pubmed-meshheading:10024343-Quaternary Ammonium Compounds, pubmed-meshheading:10024343-Synaptic Vesicles, pubmed-meshheading:10024343-Temperature, pubmed-meshheading:10024343-Time Factors
pubmed:year
1999
pubmed:articleTitle
The optically determined size of exo/endo cycling vesicle pool correlates with the quantal content at the neuromuscular junction of Drosophila larvae.
pubmed:affiliation
Institute for Behavioral Sciences, Gunma University School of Medicine, 3-39-22, Showa-machi, Maebashi, 371-8511, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't