Linked Life Data

10022430

Source:http://linkedlifedata.com/resource/pubmed/id/10022430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-2-25
pubmed:abstractText
Early human trophoblast shows dramatic invasive properties during early pregnancy. The simultaneous synthesis of matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs) in both human trophoblast and decidual membranes suggests that their controlled and balanced expression is crucial for the rapid matrix remodeling and controlled invasion during early pregnancy. Recently, we have described the presence of an extrahypothalamic GnRH immunologically, biologically and chemically identical to the hypothalamic hormone in periimplantation human embryos. Moreover, the production of this decapeptide by the human trophoblast during the early stages of placentation is well documented. TIMP-1 and -3 messenger ribonucleic acid (mRNA) expression in cultured stromal cells and protein secretion into the medium were significantly decreased by GnRH agonist compared to that in control groups. Moreover, expression of TIMP-1 was affected to a greater extent than that of TIMP-3. GnRH antagonist ablated the down-regulation of TIMPs by the GnRH agonist. MMP-9 mRNA expression was not detected in the control groups or in the groups treated with GnRH analogs. Our results provide evidence that trophoblastic GnRH may play an important role in placental tissue organization and in the early embryo-maternal dialogue by enhancing trophoblast invasion through the specific inhibition of TIMPs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
636-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10022430-Adult, pubmed-meshheading:10022430-Blotting, Western, pubmed-meshheading:10022430-Collagenases, pubmed-meshheading:10022430-Culture Media, Conditioned, pubmed-meshheading:10022430-Embryo Implantation, pubmed-meshheading:10022430-Endometrium, pubmed-meshheading:10022430-Female, pubmed-meshheading:10022430-Gene Expression Regulation, pubmed-meshheading:10022430-Gonadotropin-Releasing Hormone, pubmed-meshheading:10022430-Humans, pubmed-meshheading:10022430-Matrix Metalloproteinase 9, pubmed-meshheading:10022430-Polymerase Chain Reaction, pubmed-meshheading:10022430-Pregnancy, pubmed-meshheading:10022430-Prolactin, pubmed-meshheading:10022430-RNA, Messenger, pubmed-meshheading:10022430-Stromal Cells, pubmed-meshheading:10022430-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:10022430-Tissue Inhibitor of Metalloproteinase-3
pubmed:year
1999
pubmed:articleTitle
Independent regulation of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 (TIMP-1), and TIMP-3 in human endometrial stromal cells by gonadotropin-releasing hormone: implications in early human implantation.
pubmed:affiliation
Department of Gynecology and Obstetrics, Stanford University School of Medicine, California 94305, USA. cegiob@interbook.net
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't