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Biotin is an essential cofactor in a variety of carboxylation reactions. It is abundant in the human diet, and can be taken up from the intestinal lumen by the SLC5A6 transporter (Zempleni et al. 2009). Its covalent attachment to carboxylase enzymes is catalyzed by the HLCS enzyme in a reaction to be annotated in a future release of Reactome.
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