Source:http://www.reactome.org/biopax/48887BiochemicalReaction5210
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In the first step of PAPS biosynthesis, ATP and sulfate react to form adenylyl sulfate and pyrophosphate. Even accounting for the free energy realized from the subsequent hydrolysis of the pyrophosphate, this reaction is energetically unfavorable.
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Activation of inorganic sulfate forms APS
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ATP + sulfate => adenylyl sulfate (APS) + pyrophosphate
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2.7.7.4
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