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27-hydroxycholesterol, NADPH + H+, and O2 react to form cholest-5-ene-3beta,7alpha,27-triol, H2O, and NADP+. This reaction is catalyzed by CYP7B1 in the endoplasmic reticulum membrane. Defects in CYB7B1 are associated with failure of 7alpha-hydroxylation in vivo, and with liver damage, confirming both the function of the enzyme and the central role of the liver in this metabolic process (Setchell et al. 1998)., Authored: Jassal, B, 2007-01-19 10:34:59, Edited: D'Eustachio, P, 2007-04-30 14:43:26, Reviewed: D'Eustachio, P, 2007-04-30 14:43:26
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27-hydroxycholesterol is 7alpha-hydroxylated
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27-hydroxycholesterol + NADPH + H+ + O2 => cholest-5-ene-3beta,7alpha,27-triol + H2O + NADP+
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