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Authored: Heldin, CH, Moustakas, A, Huminiecki, L, Jassal, B, 2006-02-02, Edited: Jassal, B, 2012-04-10, Reviewed: Huang, Tao, 2012-05-14, SMURF2, an E3 ubiquitin protein ligase, binds to SMAD7 in the nucleus. WW2 and WW3 domains of SMURF2 are both required for binding PY motif (PPXY sequence) of SMAD7. Endogenous human SMAD7 and SMURF2 were shown to form a complex in human U4A/Jak1 cells, derived from a sarcoma cell line 2fTGH. The interaction was studied in more detail by expressing tagged recombinant human SMURF2 and mouse Smad7 in human embryonic kidney cell line HEK293 (Kavsak et al. 2000, Ogunjimi et al. 2005).
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SMAD7 binds to SMURF2
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