The enzyme catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation.
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PafA is required to confer resistance against the lethal effects of reactive nitrogen intermediates (RNI), antimicrobial molecules produced by activated macrophages and other cell types.,
The enzyme catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation.,
The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.,
This tagging system is termed pupylation.
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| uniprot:name |
Pup--protein ligase,
Pup-conjugating enzyme
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| uniprot:activity |
ATP + [Pup] + [protein]-lysine = ADP + phosphate + [protein]-N-pupyllysine.
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