The SAGA histone acetyltransferase/transcriptional adaptor complex is composed of multiple transcriptional regulators including Ada, Spt, and TAFII proteins. Here we identify an additional novel subunit of the complex, Tra1, an ATM/PI-3-kinase-related homolog of the human TRRAP cofactor, which is essential for c-Myc and E2F-mediated oncogenic transformation. Mass spectrometry, immunoblotting, and immunoprecipitation experiments confirm the stable association of this protein within SAGA. In addition, the Tra1 protein is a component of at least two other histone acetyltransferase protein complexes. These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The SAGA histone acetyltransferase/transcriptional adaptor complex is composed of multiple transcriptional regulators including Ada, Spt, and TAFII proteins. Here we identify an additional novel subunit of the complex, Tra1, an ATM/PI-3-kinase-related homolog of the human TRRAP cofactor, which is essential for c-Myc and E2F-mediated oncogenic transformation. Mass spectrometry, immunoblotting, and immunoprecipitation experiments confirm the stable association of this protein within SAGA. In addition, the Tra1 protein is a component of at least two other histone acetyltransferase protein complexes. These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter.
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| skos:exactMatch | |
| uniprot:name |
Mol. Cell
|
| uniprot:author |
Grant P.A.,
Pray-Grant M.G.,
Schieltz D.,
Workman J.L.,
Yates J.R. III
|
| uniprot:date |
1998
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| uniprot:pages |
863-867
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| uniprot:title |
The ATM-related cofactor Tra1 is a component of the purified SAGA complex.
|
| uniprot:volume |
2
|
| dc-term:identifier |
doi:10.1016/S1097-2765(00)80300-7
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