In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin alpha. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin alpha. Although the encoded protein had only 23-27% amino acid identity to the importin alphas from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin alpha in plants.
| Predicate | Object |
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| rdf:type | |
| rdfs:comment |
In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin alpha. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin alpha. Although the encoded protein had only 23-27% amino acid identity to the importin alphas from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin alpha in plants.
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| skos:exactMatch | |
| uniprot:name |
FEBS Lett.
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| uniprot:author |
Iwasaki T.,
Matsuki R.,
Miyao M.,
Sanmiya K.,
Shoji K.,
Yamamoto N.
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| uniprot:date |
1998
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| uniprot:pages |
259-262
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| uniprot:title |
A novel importin alpha from rice, a component involved in the process of nuclear protein transport.
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| uniprot:volume |
428
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| dc-term:identifier |
doi:10.1016/S0014-5793(98)00540-7
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