Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis.
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| skos:exactMatch | |
| uniprot:name |
Biochem. Biophys. Res. Commun.
|
| uniprot:author |
Liu M.-C.,
Nakayama T.,
Sakakibara Y.,
Suiko M.,
Takami Y.,
Yanagisawa K.
|
| uniprot:date |
1998
|
| uniprot:pages |
681-686
|
| uniprot:title |
Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases.
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| uniprot:volume |
247
|
| dc-term:identifier |
doi:10.1006/bbrc.1998.8872
|