The thiA gene is involved in the biosynthesis of the pyrimidine moiety of thiamin in Bacillus subtilis. This gene was cloned using a DNA probe rescued from thiA::Tn917 to screen a B. subtilis genomic library. ThiA exhibits 70% identity with E. coli thiC. The proposed thiA open reading frame complements all available thiA mutants of B. subtilis as well as the thiC mutant of E. coli. This suggests a similar biosynthetic pathway to 2-methyl-4-amino-5-hydroxymethylpyrimidine in both organisms. The expression of thiA is regulated, at least on the transcriptional level, and is significantly repressed by thiamin and 2-methyl-4-amino-5-hydroxymethylpyrimidine.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The thiA gene is involved in the biosynthesis of the pyrimidine moiety of thiamin in Bacillus subtilis. This gene was cloned using a DNA probe rescued from thiA::Tn917 to screen a B. subtilis genomic library. ThiA exhibits 70% identity with E. coli thiC. The proposed thiA open reading frame complements all available thiA mutants of B. subtilis as well as the thiC mutant of E. coli. This suggests a similar biosynthetic pathway to 2-methyl-4-amino-5-hydroxymethylpyrimidine in both organisms. The expression of thiA is regulated, at least on the transcriptional level, and is significantly repressed by thiamin and 2-methyl-4-amino-5-hydroxymethylpyrimidine.
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| skos:exactMatch | |
| uniprot:name |
Gene
|
| uniprot:author |
Begley T.P.,
Zhang Y.
|
| uniprot:date |
1997
|
| uniprot:pages |
73-82
|
| uniprot:title |
Cloning, sequencing and regulation of thiA, a thiamin biosynthesis gene from Bacillus subtilis.
|
| uniprot:volume |
198
|
| dc-term:identifier |
doi:10.1016/S0378-1119(97)00295-3
|