The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.
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| skos:exactMatch | |
| uniprot:name |
DNA Res.
|
| uniprot:author |
Aiba H.,
Baba T.,
Fujita K.,
Hayashi K.,
Horiuchi T.,
Inada T.,
Isono K.,
Itoh T.,
Kasai H.,
Kimura S.,
Kitagawa M.,
Kitakawa M.,
Makino K.,
Miki T.,
Mizobuchi K.,
Mori H.,
Mori T.,
Motomura K.,
Nakade S.,
Nakamura Y.,
Nashimoto H.,
Nishio Y.,
Oshima T.,
Saito N.,
Sampei G.,
Seki Y.,
Sivasundaram S.,
Tagami H.,
Takeda J.,
Takemoto K.,
Wada C.,
Yamamoto Y.
|
| uniprot:authorsIncomplete |
true
|
| uniprot:date |
1996
|
| uniprot:pages |
379-392
|
| uniprot:title |
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
|
| uniprot:volume |
3
|
| dc-term:identifier |
doi:10.1093/dnares/3.6.379
|