Following the cloning of the dopamine receptors we continued a search of the human genome for related genes. We searched an EST data base and discovered cDNA fragments encoding novel G protein-coupled receptor genes. The available GenBank sequence of one of these EST fragments showed that it encoded a receptor with closest similarity to the D2 dopamine and adrenergic receptors. This cDNA was used to isolate the gene (GPR19), and the encoded receptor also demonstrated similarity with the neuropeptide Y receptor. The gene was mapped to chromosome 12, in region p13.2-12.3. Northern blot analysis revealed expression of GPR19 in peripheral regions, and brain regions significantly overlapping with the D2 receptor gene expression. A sequence of the rat orthologue of GPR19 was obtained and in situ hybridization analysis demonstrated a very abundant expression in rat brain.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
Following the cloning of the dopamine receptors we continued a search of the human genome for related genes. We searched an EST data base and discovered cDNA fragments encoding novel G protein-coupled receptor genes. The available GenBank sequence of one of these EST fragments showed that it encoded a receptor with closest similarity to the D2 dopamine and adrenergic receptors. This cDNA was used to isolate the gene (GPR19), and the encoded receptor also demonstrated similarity with the neuropeptide Y receptor. The gene was mapped to chromosome 12, in region p13.2-12.3. Northern blot analysis revealed expression of GPR19 in peripheral regions, and brain regions significantly overlapping with the D2 receptor gene expression. A sequence of the rat orthologue of GPR19 was obtained and in situ hybridization analysis demonstrated a very abundant expression in rat brain.
|
| skos:exactMatch | |
| uniprot:name |
FEBS Lett.
|
| uniprot:author |
Cheng R.,
George S.R.,
Heng H.H.Q.,
Kolakowski L.F. Jr.,
Lynch K.R.,
Marchese A.,
Ng G.Y.K.,
Nguyen T.,
O'Dowd B.F.,
Thompson M.
|
| uniprot:date |
1996
|
| uniprot:pages |
325-329
|
| uniprot:title |
A novel gene codes for a putative G protein-coupled receptor with an abundant expression in brain.
|
| uniprot:volume |
394
|
| dc-term:identifier |
doi:10.1016/0014-5793(96)00901-5
|