The guanylyl cyclase C protein, expressed primarily in the intestine, is the receptor for the heat-stable enterotoxin of Escherichia coli. We have isolated and sequenced the promoter region and the first exon of human guanylyl cyclase C and determined the major site of transcription initiation. Transfection of a -1973/+124 promoter/luciferase gene fusion construct in the Caco-2 intestinal cell line resulted in a high level of expression; results with deletion constructs indicate the presence of multiple positive-acting sequence elements. These promoter elements were not active upon transfection into NIH/3T3 and LLC-PK1 cell lines which do not express GC-C.
| Predicate | Object |
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| rdf:type | |
| rdfs:comment |
The guanylyl cyclase C protein, expressed primarily in the intestine, is the receptor for the heat-stable enterotoxin of Escherichia coli. We have isolated and sequenced the promoter region and the first exon of human guanylyl cyclase C and determined the major site of transcription initiation. Transfection of a -1973/+124 promoter/luciferase gene fusion construct in the Caco-2 intestinal cell line resulted in a high level of expression; results with deletion constructs indicate the presence of multiple positive-acting sequence elements. These promoter elements were not active upon transfection into NIH/3T3 and LLC-PK1 cell lines which do not express GC-C.
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| skos:exactMatch | |
| uniprot:name |
Biochim. Biophys. Acta
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| uniprot:author |
Giannella R.A.,
Jump M.L.,
Mann E.A.
|
| uniprot:date |
1996
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| uniprot:pages |
7-10
|
| uniprot:title |
Cell line-specific transcriptional activation of the promoter of the human guanylyl cyclase C/heat-stable enterotoxin receptor gene.
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| uniprot:volume |
1305
|
| dc-term:identifier |
doi:10.1016/0167-4781(95)00190-5
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