The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.
|
| skos:exactMatch | |
| uniprot:name |
Nucleic Acids Res.
|
| uniprot:author |
Aebersold R.H.,
Carter J.R.,
Franden M.A.,
Kim D.R.,
McHenry C.S.
|
| uniprot:date |
1993
|
| uniprot:pages |
3281-3286
|
| uniprot:title |
Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme.
|
| uniprot:volume |
21
|
| dc-term:identifier |
doi:10.1093/nar/21.14.3281
|