A full length ovine steroid 11 beta-hydroxylase (cytochrome P-450(11 beta)) cDNA clone from a sheep adrenal cortex cDNA library was isolated. Sequence analysis indicates that this cDNA clone resembles bovine P-450(11 beta) cDNA (95% nucleotide sequence homology) more closely than rat P-450(11 beta) cDNA (69% nucleotide sequence homology). Although the levels of nucleotide sequence homology of this cDNA clone to the rat P-450(11 beta) cDNA and the rat P-450aldo cDNA are similar, the putative amino acid sequence shows a closer resemblance to rat P-450aldo protein. Northern blot analysis shows that there are three sizes of transcript and they are expressed throughout the adrenal cortex.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
A full length ovine steroid 11 beta-hydroxylase (cytochrome P-450(11 beta)) cDNA clone from a sheep adrenal cortex cDNA library was isolated. Sequence analysis indicates that this cDNA clone resembles bovine P-450(11 beta) cDNA (95% nucleotide sequence homology) more closely than rat P-450(11 beta) cDNA (69% nucleotide sequence homology). Although the levels of nucleotide sequence homology of this cDNA clone to the rat P-450(11 beta) cDNA and the rat P-450aldo cDNA are similar, the putative amino acid sequence shows a closer resemblance to rat P-450aldo protein. Northern blot analysis shows that there are three sizes of transcript and they are expressed throughout the adrenal cortex.
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| skos:exactMatch | |
| uniprot:name |
Biochim. Biophys. Acta
|
| uniprot:author |
Boon W.C.,
Coghlan J.P.,
Crawford R.J.,
Hammond V.E.,
Jeyaseelan K.,
Roche P.J.
|
| uniprot:date |
1995
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| uniprot:pages |
109-112
|
| uniprot:title |
Cloning and expression analysis of a cytochrome P-450(11 beta) cDNA in sheep.
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| uniprot:volume |
1260
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| dc-term:identifier |
doi:10.1016/0167-4781(94)00190-E
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