Using oligodeoxyribonucleotide primers and the polymerase chain reaction, we cloned and sequenced a 3.2-kb region which contains the gyrB gene, encoding the beta-subunit of gyrase from Mycoplasma capricolum. We localized gyrB opposite from the chromosomal replication origin (ori) of M. capricolum by genomic Southern hybridization.
| Predicate | Object |
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| rdf:type | |
| rdfs:comment |
Using oligodeoxyribonucleotide primers and the polymerase chain reaction, we cloned and sequenced a 3.2-kb region which contains the gyrB gene, encoding the beta-subunit of gyrase from Mycoplasma capricolum. We localized gyrB opposite from the chromosomal replication origin (ori) of M. capricolum by genomic Southern hybridization.
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| skos:exactMatch | |
| uniprot:name |
Gene
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| uniprot:author |
Miyata M.,
Sano K.
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| uniprot:date |
1994
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| uniprot:pages |
181-183
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| uniprot:title |
The gyrB gene lies opposite from the replication origin on the circular chromosome of Mycoplasma capricolum.
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| uniprot:volume |
151
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| dc-term:identifier |
doi:10.1016/0378-1119(94)90653-X
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