Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.
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Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCM1). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.
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| uniprot:name |
Acta Biochim. Pol.
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| uniprot:author |
Augustyniak J.,
Malinowski T.,
Misztal L.,
Musiao W.G.,
Pater B.,
Wypijewski K.J.
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| uniprot:date |
1995
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| uniprot:pages |
97-101
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| uniprot:title |
Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants.
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| uniprot:volume |
42
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